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The in vivo integration of linear retroviral DNA catalyzed by integrase occurs by a concerted mechanism known as full-site or two-ended integration. The purified recombinant enzyme also accurately reproduces concerted integration, but the in vitro efficiency is still far below that expected for integration in vivo. Early reconstitution experiments using purified IN, a linear retrovirus-like DNA donor and a target DNA were successful but yielded low levels of concerted integration 1 , 2.

The efficiency of full-site integration was significantly increased later on by employing non-ionic detergent IN-containing lysates of HIV-1 virions 3 , but the proficiency and reliability of the reaction vary with the various reconstitution systems 4. Although purified HIV-1 IN can perform full-site integration in vitro , retroviral proteins, such as the nucleocapsid protein NCp 5 , or factors, such as HMGA1, belonging to cellular high mobility group proteins have been shown to mediate efficient full-site integration 6.

The lack of these factors in in vitro integration assays may account for the differences observed. Moreover, deficient protein-folding or incorrect oligomerization of purified IN may also prevent native assembly of complexes with viral DNA ends, which is essential for full-site integration 7 , 8. The aim of our study was to determine whether efficiency of the full-site integration activity is correlated with the oligomerization state of IN.

For that purpose we stabilized the multimeric structure of the enzyme by chemical crosslinking. We determined the activity of the isolated different IN monomers and multimers and observed that the cross-linked tetrameric form of IN is the minimal oligomer that is able to perform full-site integration of a substrate carrying the two LTRs. DNA was extracted and purified as previously described 9. HIV-1 IN was expressed in yeast and purified as previously described Proteins were eluted with a flow rate of 0.

The protein composition of the pooled fractions was confirmed by mass spectrometry. The concerted integration reaction conditions were similar to those described in reference 13, except that no cellular proteins were added and the HIV-1 system was used. IN activity was quantified by scanning the bands using the NIH software. All IN activities were quantified by scanning of the bands after gel electrophoresis and autoradiography using the NIH software. Both ampicillin and tetracycline resistance genes carry an amb mutation. These proteins are thus expressed only in the presence of the supF gene products.

The platinum derivative was prepared as described before To determine whether the yeast recombinant IN was able to catalyze the in vitro concerted integration reaction, the purified enzyme was assayed as described in the Material and Methods section. The donor—target products were visualized by agarose gel electrophoresis using radioactively labeled donors. This shows that this enzyme was less efficient for performing the whole integration reaction than the purified preintegration complex PIC or INs from other retroviral sources 3 , A Gel analysis of the integration products.

SI: Self-integration products. M: Markers base pairs. The structure of the expected products are also described in this figure. The percentage of the total integrated substrate was reported on the graph. Restriction analyses were performed to assess the nature of the products data not shown. Form I comprises circular molecules resulting from the integration of one LTR one-site or non-concerted integration, nCI and of two LTRs concerted integration, CI from the same donor into the receptor plasmid.

Form II corresponds to linear full-site concerted integration products of two LTRs from two donor molecules into the receptor plasmid. Since form I includes the sum of different integration products concerted and non-concerted and all the linear products found in form II may be considered as originating from concerted integration, the latter form was considered as representative of integration of two LTR into the target DNA. Gel analysis permits quantification of global integration efficiency but does not make it possible to distinguish one-site insertion from the concerted integration products.

Only DNA products carrying the amber mutation suppressor gene supF should be able to replicate and form colonies under drug selection. Thus, only the circular concerted integrated products corresponding to form I should replicate in bacteria. In contrast, no colonies were selected when the integration reaction was performed using an inactive IN that exhibits the DA mutation.

We sequenced the donor DNA-acceptor plasmid junctions of the isolated integration products to check the accuracy of the integration reaction duplication of short acceptor DNA sequence. As reported in Table 1 , of 20 clones, 12 exhibited a target DNA duplication of 5 bp, the hallmark of HIV-1 IN in vivo integration activity, 6 clones showed a duplication of different sizes and 2 clones carried a short deletion of the target DNA.

These results indicate that INs purified from yeast shares the same in vitro characteristics as other recombinant INs obtained from bacteria 15 , Sequence analysis of donor—target junctions from clones produced by wild type IN. This could be due to: i a need for auxiliary, viral or cellular proteins that are absent from the in vitro preparation; ii differences between the specific activities of virion and recombinant integrases and iii inefficient folding or oligomerization of the enzyme.

We investigated this by studying the possible link between IN multimerization and its in vitro integration activity and the in vitro activity of pre-stabilized IN oligomers. To address the question of the enzymatic activity versus the oligomerization state of HIV-1 IN, the enzyme was crosslinked to form stable multimers. The chemical crosslinking agent used was cis -aquahydroxydiamino platinum.

This technique allows the formation of covalent bonds between platinum and the potential acceptors on proteins and nucleic acids, mainly the sulfur-containing groups of cysteine or methionine residues, the imidazole rings in histidine residues, and the N group of guanine, adenine and cytosine The elution profile of the different forms of IN was checked with known molecular weight protein markers Figure 2A. Before the crosslinking treatment IN appeared in solution mainly as a monomer—dimer equilibrium while a lower but significant amount of tetramer was detected Figure 2A , 0 min indicating that our yeast recombinant IN has the same oligomeric structure in solution as recombinant bacterial IN 18 , As expected, the time duration of the crosslinking treatment increased the proportion of oligomeric forms of the enzyme including tetramers of IN Figure 2A , 45 and min.

High molecular weight aggregates were eliminated through centrifugation before loading onto the column. IN pmol was crosslinked for different lengths of time and products were loaded on a Superose 12 column. Migration of the molecular weight markers is indicated. B Size of the IN oligomers determined by gel filtration chromatography. The elution position of each fraction was reported shown as triangles in this figure and compared to elution profile of known molecular weight markers shown as clubs in the figure : catalase kDa , bovine serum albumin 67 kDa , ovalbumin 43 kDa and chymotrypsinogen A 25 kDa.

To obtain stable oligomers we performed a min incubation of IN in the presence of AHDAP and chromatographed through the Superose column allowing sufficient separation in order to isolate tetramers, dimers and monomers. Fractions containing the different oligomeric forms of IN were collected and their nature was checked by loading an aliquot of each fraction onto a new Superose column.

As reported in Figure 2B the fractions migrated as expected from their corresponding oligomeric size. Purified fractions of crosslinked IN oligomers were obtained and stocked at sub-micromolar protein concentrations from to nM. Those concentrations were maintained during all subsequent studies. Since under these conditions wild type IN appears mainly as a monomer, as described previously by time-resolved fluorescence anisotropy 18 , no re-association of the purified crosslinked oligomers was expected to take place during further steps of this study.

The fractions were tested for concerted integration Figure 3A. We focused our interest on integration products I and II since they are the most representative of the physiological integration reaction. Interestingly, amongst the crosslinked forms of IN, only the fraction containing tetramers lane IN Te was able to catalyze the formation of the full-site concerted integration form II product.

A control with IN that was not crosslinked lane IN NC showed that this fraction was also able to perform the concerted integration reaction in addition to non-concerted integration. Integration products were further analyzed by cloning them into bacteria. As reported in Figure 3B , a mean value of 25 clones were recovered from the cloning of the integration products obtained with non-crosslinked IN and 70 with crosslinked tetramers indicating a higher level of concerted integration catalyzed by the tetramer of IN as compared with the non-crosslinked protein.

In order to identify the structure of the integration products, 20 clones were isolated and sequenced for each condition Table 2. From the 20 clones obtained with non-crosslinked IN, 11 exhibited a target DNA duplication of 5 bp as expected from HIV-1 IN activity, 6 exhibited a duplication comprising sizes from 3 to 6 bp and 3 exhibited a deletion in the acceptor DNA.

In sharp contrast, all the clones obtained with products of the reaction catalyzed by the IN tetramers exhibited the HIV-1 IN 5 bp duplication hallmark. This striking result strongly supports the idea that crosslinked IN tetramers display a higher concerted integration activity than non-crosslinked IN tetramers thereby suggesting the involvement of the tetrameric forms in the physiological integration of both LTR viral ends in the infected cell.

Sequence analysis of donor—target junctions from clones produced by non-crosslinked and crosslinked tetrameric IN. Plasmid DNA from the selected clones was purified and sequenced as described in Materials and Methods. Both processing and strand transfer activities were detected only with the dimeric form of IN. Taken together these results show that the different oligomeric forms of IN do not share the same in vitro catalytic properties. Monomers were inactive for all specific IN activities. In contrast, the full-site integration of both LTR extremities required at least one tetramer. The requirement of high concentrations of non-physiological molecules like PEG and DMSO to obtain efficient in vitro integrations catalyzed by wild type IN NC suggests that this recombinant protein might need the presence of these agents to attain a significant degree of activity.

To investigate this hypothesis, we tested the effect of these compounds on the full-site integration reaction catalyzed with the purified IN Te. Effect of reaction conditions on concerted integration activity. These results indicate that: i the tetrameric form of IN possesses biochemical properties closer to the enzyme found in the infected cell than to the native non-crosslinked IN preparation and ii DMSO could compensate for deficient oligomerization of the non-crosslinked IN required for efficient in vitro integration.

In contrast, PEG may act directly on the integration process most probably via a molecular crowding effect. Thus, we used the crosslinking approach to analyze the oligomeric forms of the enzyme bound to different DNA substrates. The products were subjected to DNase I treatment, in order to eliminate non-IN-protected DNA tails and to distinguish between monomers and higher oligomeric forms of IN bound to the oligonucleotide.

High molecular weight multimeric products unable to enter the gel were obtained when IN was crosslinked to the DNA oligonucleotide without a viral sequence Figure 5A. The first band, termed a , which presents a size that corresponds to a monomer of IN, was observed before the formation of the second IN—DNA complex, termed b. Ported to meet the giant requ Extended Properties. This addon adds a lot of properties to Half-Life 2 and Garry's Mod entites such as turrets, rollermines, suit chargers, flashlights, dynamites, NPCs and anti-antlion thumpers.

There are over 72 different actions for different entites and NPCs. Extended Spawnmenu. Extra Customizable Weaponry 2. Created by [ES] Bub. FA:S 2. This is the official FA:S 2. The base is written completely from scratch. In case you're interested, get t [www. Requires FA:S 2. Click here Requires FA:S 2. Fading door tool. Created by Panthera Tigris. A tool that makes anything into a fadable door. Features - Can select material. Fading Doors - Original. Created by SiKa.

Simply press that subscribe button to get it. Those instructions below, is only if you're having it on your server. Created by MARK This port is in my opinion the most successful versions of Alyx Vance from FakeFactory mod. Port is made by me, also added hands from 1st person. The original models - Fak [cinematicmod. Falco's famous prop protection, FPP.

Fallout 4 - Facial Hair Pack. Created by sir dickmosi. Fallout Shelter. Created by FroZeXminerX. FBI Playermodels. Fello's SWEPs 2. Created by CmndrFello. Femscout's Improved Bodygroups [Paintable]. FF2 Jeff the Killer. Eyes and face are also not posable.

FF2 Jeff Created by Michael He adventures with his brother Jake the Dog Who is yet to be added! As a baby, he was abandoned in the woods on a leaf as a baby before being picked u Five Nights at Freddy's - Playermodels. These are the five nights at freddy's models that i6nis made for slender fortress, but with revamped shaders, and on the valve biped, so they work as playermodels they all have viewmodel hands too knock yourselves out credits to i6nis for making the m Created by pillow. To View Updates, please click here. Please read the description before commenting.

Five Nights at Freddy's Animatronics. Created by Splinks. Hello, hello? Seems you've made an interesting stumble; let me fill you in. Freddy and his friends are are here and at your control. Think of them as your puppets That is if you only mess with the ragdolls. Five Nights at Freddy's Gamemode. Created by VictorienXP. The gamemode goal is to improve the gaming experience on the FNaF map! Fixed Danboard Player Model Created by blim ;.

Ok so I know this is already on the workshop but, I've given it more than 4 months to be fixed and something that took me 5 minutes to fix couldn't be done apparently So here you go to you die hard danboard fans. Go crazy Flight SWEP. Created by tuxe. A semi realistic flight simulator that I made while learning lua.

Turned out to be much more fun that anticipated. Primary Fire Left click starts flight mode, where you can essentially walk in any direction, even up. You cannot take fall damage while Food and Household items. This pack contains well over props, mainly food but also cleaning products and other types of groceries. Note: if you don't have tf2 for you Freeman's "Model a Day" Pack. Created by The One Free-Man. Here's a pack of models I made over the summer because I was bored. Everything here was suggested in a thread on Facepunch, and all the props in this pack where made in less than a day each.

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Created by wii. Gabe the Dog Meme Run. For use in Meme run Bark!!! The Meme community is devastated by the loss of Gabe the Dog so i made this to remember him by. His barking and growling will get increasingly louder the closer he is to you If it does not work type in console: sv Garry's mod 10 Counter-Strike: Source Weapons. Garry's Mod Construct Gas Can. Created by Fulfilled Archway. Created by finland. Created by Kogitsune.

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Start fires. Bring a burning alter of the inferno into creation. Created by Littlefoot. Created by Tenrys. This addon adds a new sub-menu available in the Context Menu, named ''Gestures''. Clicking any option will make you perform a Gesture, like dancing, or agreeing. Ghost Rider Playermodel. Created by Rokay. Gibby Nextbot. Created by Capitalism. Global Cross Player Models. Glock Collection. GM Metropolys. Gmod Death View. Created by Valkyrie. Gmod Legs 3. Created by MacDGuy. Yes, they are compatible with Sandbox.

Most player models have coloring support unique to their original model Created by -Bn- Vamp. Facepunch Thread [facepunch. Created by Zombie Kenny. Created by QuickNinjaCat. I had taken the old one down due to it not updating properly. This addon features: A map with a sandbox, a grassbox, and a snowbox, a map icon This is my first map, also this map using to test addons or dupes :D Created by Bigwig. A post-nuclear gmod map. Fully noded for AI If you are seeing checkered shadows on the ground it's because you don't have Episode 2 installed.

Created by andeski. Created by InsaneAlternative. My YouTube! Huge Revamp of the map! Newer, lovelier textures Fixed broken textures Added water pool 3D skybox Tower is better This map is nextbot ready and has a nevmesh. Created by Greensl. Features: -Spawn hub with teleporters -Big hole in the ground for dropping stuff -An aboveground water area -A Created by sub This is a special-purpose map designed for drag racing and vehicle testing.

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An inlet on a pacific island. This is my first mapping project so it has a bit of an identity crisis. It has an areaportaled build area. Based on the northern shore of the island of Guadalcanal. You ma Created by Drunk Sailor. Created by Mr. Created by LegendofRobbo. Created by bazylglowacki Key-Drop.

This is to stop people from having SHA-1 Issues, contact me for more info Created by Matchy. This map is made by Sportkeks! Created by Iron Roadkill. Be sure to comment and leave a like! I would like to introduce my second map, It consists of large mountains with custom textures, as well as some small buildings. I generated a x heightmap in world machine then imported it into hammer with dispge Created by T9P. Watri has made this map and was paid by Fire Pug to do so. A map that I am working on. It's a work in progress, and the map name changes everytime I release a revision. Due to this, it may not be suitable to use on a server.

The entire series of versi Created by HUNcamper. Created by markrosy Created by PotatoKittens. Created by Sentient Fungi. Created by hiro. This is reupload of a map i'v found on garrysmod. Test Island is a little island with quite enough build space, and a few different surfaces made for material testing purposes. Includes navmesh! Created by WeenieHutJunior. Created by Lewd. World with many secrets and cool areas to explore and discover. Created by David Ralphsky. First of all, I fixed all of the server-side script errors for everything. Gods Little Finger.

Created by Chowder TeamCorgi. Golec Brothers Playermodels. Created by Tuscan These are Golec Brothers from Golec uOrkiestra in their own persons. Now you can play as them on Garry's Mod! Gordon Freeman. Created by Paynamia. Gordon model with Romka's PS2 remake head and modified Romka body. Uses new eye shaders.

NPC, Playermodel, Ragdoll. Model now supports faceposer! The face can also be morphed even further via the Joint Tool. Includes five different sets of glasses, as w Gordon Freeman by DPFilms. Gordon Freeman Player Model. Gordon Freeman Player models. Created by gaelcoral. Gordon Freeman Playermodel. I was searching for good Gordon model for a long time until i found this one in Source Film Maker's Grappling Hook. Created by Jvs. It's a grappling hook!

Press use on it and hold G on your keyboard to use it. Hold C and right click edit the grappling hook to change the keybind, it doesn't save the button right now but it will in the future. Grizzly's Personal Player Model. Created by Grizzly. My own personal player model! GTA 5 Playermodels. Created by Dusty. Features: Trevor is shit, no fingerposing and he's got a colorable shirt. Michael's got 9 skingroups for his suit, and a colorable dress shirt.

Franklin is probably the most complete. GTA Player Models. Guess Who. Created by lolle. H2O Delirious Player Model. Here it is, a H20 Delirious Player Model. Created by Speedwagon. Created by DobriiVecher. Haax Gun 2.

Star Force: Integration

Created by Ome Joey. Press left mouse to fire a single computer and right mouse to unleash the rapid-fire mode! If you have any suggestions on what to add next, please leave a comment below! Known issues: - none SWEP V2. Created by SL G J4k3. The HAAX!!! Take care of those pesky cheaters in a fun way Created by Battlepope. Half-Life 2: Survivor Player Models. Usable player models from Half-Life 2: Survivor. Created by Leafdroid. A cool addon that adds sweps wich allows you to do some cool moves!

At the moment the animations only look right when u stand still! A tool that adds paint to TF2 cosmetics, and another that adds critglow or jarate effects to TF2 items and characters. Requires Team Fortress 2 for crit spark and jarate drip effects. Created by pEEbz. Created by da31o. Created by Bone Head. Heartless Physics Gun Skin. Created by ckosmic. This is a texture for your physics gun. It's the heartless emblem from Kingdom Hearts Hide and Seek. It does not contain; stock rtv, dynamic tag-ranges, anti-cheat. It still includes achievements and required fixes.

HL2 vehicles with guns. Created by yackson. Airboat and Jeep with added guns, like in HL2. It only adds HL: Gordon Freeman P. Model used by Hunt Down The Freeman is a different version. For to respect all the creators credited below, their work has not been used in HDTF version. It's Gordon Freeman! Horror Story. Created by SirQuack. Ever wanted to play horror maps without having to worry about noclipping co-players and use of weapons they shouldn't use?

Then this is the addon you need. Horror Story turns Garry's Mod into a full-fledged, fully compatible horror game. Features like Hotline Miami's Jacket. You have a new message! Viewer discretion advised. All suspects are innocent until p Please hang up an Created by Dziubak Cartman.

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My house :D You enter the door number 7 and then enter the building, go to the 3rd floor, and it is so cool room Hoverboards is a reboot of 's quite popular mod for Garry's Mod. It was originally created by Foszor and Jinto. This pack also includes some props for your enjoyment. Usage Information. Huntsman Knife Collection. Huseey Nextbot. Created by -Brittank Huseey, now as a meme nextbot. I've hit new lows and new highs at the same time Created by Joshuggah! Infinite ammo supplys for m9k.

Created by Cancer. Created by. Credit to whoever made the original. Instant Keypad Cracker. Created by Megamezzomixer. Are you an admin and just want to test out if a Keypad is wired correctly? And are you tired of waiting so long until the keypad is finally cracked? Then this addon is just for you! If you like that addon, please don't forget to leave a like.

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Iron Man SWep. Created by FrostyAvo. Iron Man's Mark 7 armor, used as his primary suit in The Avengers. The Iron Patriot's armor, as seen in Iron Man 3. What's in it: - Two NPCs for each set of armor, good and bad. Sorry, they just use the standard rebel and combine base - no flying, la Jane The Killer Jane Everlasting. Created by TrapQueenThingz. This is my custom model. It's Jane the Killer Jane Everlasting! Now Jeff's got company! Credits: Bloocobalt - Dress Model porting L Jacket Player Expression.

Created by Fiaasow. Oh wow, thumbs? I actually never would've thought this little thing was going to hit thumbs. Thanks for proving me wrong, fellas :3 I will be working on more addons in the future, just been kinda ou Created by [FR] Sadamer.


Created by xXPastamaniac43Xx. Jeff the Hunter Ragdoll. Created by SpongePierre. Created by dewobedil. On a serious note this is a CreepyPasta character I never quite understood on how people chose to visually portray and instead chose to ruin a rather potentially insanely scary figure. So I chose to give my twisted take on him by combining the fan ap Created by not Ripa Moramee. You need jeff the killer model he doesnt do much he just turns but if you guys want ican make him a turet but he will shoot froom his eyes and that is not jeff s thing i made him a combine stalker but he cant move at least he looks cool, pls subscribe,th I did not create this, and take no credit for it aside from the fixed eye texture.

Credit goes to Splinks for making everything. Jeff The Killer Pony. Created by Aqua. Jesus Playermodel. It's a jetpack! You can equip it by pressing USE default key is E on your keyboard. WASD to move, shift to hover, jump to rise up and crouch to go down. Created by shoopdahoop Created by elt kralking This addon adds a new swep called "Advanced Keypad Cracker". It can be used to hack keypads just like default Keypad Cracker, but with better models and effects Keypad Cracker. Created by Monster. This is just a regular keypad cracker, though everyone can use it, and not just admins.

Hope you enjoy! Configuation: - Incorrect attempts before Work as a player Created by A-Crow. Laser Gun. Created by techdra. Staff Online. Members Most Online Star Force series by Aer-ki jyr Posts : Reputation : 1 Join date : Country: :. You think everything is under control. You think there is nothing Humanity cannot do if it puts its mind to it. Earth is not what you think it is.

It is a ticking time bomb wrapped up in a delusional utopia called everyday life. One man discovers that past and the inevitable countdown that Earth is under. An organization known as Star Force. Star Force: Integration SF2 With their advanced training underway in Atlantis, Paul and his teammates are sent on a 'field trip' to space for zero g and high gravity training, during which they get to see and experience the wonders of the space corporation's orbital infrastructure and economy first hand.

Star Force: Nemesis SF3 Training continues with new and varied combat 'Challenges' being passed by Paul and the other elite trainees far faster than the Star Force trainers anticipated Star Force: Trailblazer SF4 With the trainees moving on through their training at an accelerated pace, the 'Challenges' branch out into new areas including land vehicles, aircraft, and space combat Star Force: Gemini SF5 The trainees come to the final stage of their training before graduation, the 'Tandem Challenges,' during which the top team and individual honors will be determined, with Paul and Morgan racing each other to collect as many points as possible before they arrive at the 'Final Challenge.

Star Force: Fabrication SF7 Paul and the other adepts start adjusting to their new roll, free of trainer oversight and control as they push their abilities past Human norms while simultaneously designing the military structure that Davis has tasked them to command. Star Force: Flashpoint SF8 Years have passed since their initial training but the existence of the Archons and Star Force's ever growing military still remains a secret from the public as Earth continues to grow its orbital and Lunar colonies. That growth, however, is not without risk and the perils of space travel are ever present, forcing Star Force into the role of shieldbearer for the nations of Earth as events force their hand and a revelation of their military might becomes inevitable.

Star Force: Mettle SF9 In the aftermath of the mysterious pirate attacks tensions rise as the identity of the nation behind the ruse is revealed and unilateral penalties are handed down Star Force: Disarmament SF10 With the brunt of the Chinese attack repelled, Star Force pushes into the enemy's Lunar territories, intent on ending the brief war by expelling the attackers from the moon entirely.

Meanwhile the naval conflict escalates, with the Chinese fleet still running dark and unaccounted for. Star Force: Deception SF11 The colonization rush is in full swing, with millions of people now living on Mars and the edge of Star Force's dominion stretching all the way out to Jupiter. Nations vie for dominance, expanding their fleets and gathering up as much territory as they can, with Star Force being the nonpartisan arbiter. Tensions are on the rise, however, as a series of mining incidents in the asteroid belt pit the Americans against the British in a growing conflict that could lead to the third war in the pair's history.

With that Earthbound indemnity nations pour their resources into battling each other while Star Force protects itself and the neutral parties, looking for a way to reign in the belligerent nations before before the conflict spreads any further Star Force: Colonization SF15 With the war behind them, Star Force pushes forward with an aggressive expansion campaign that sees the Archons' new Clans becoming splinter entities of their own right per Davis's master plan as he directs his ever growing mega corporation to the very edges of the Solar System Star Force: Canderous SF16 With the establishment of minimal trade routes to nearby star systems, Star Force begins the first stages of opening up new worlds to national colonization in the Epsilon Eridani System with a test case, only to run into more trouble than anyone anticipated.

Star Force: Intimidation SF17 Now wrapped up in an interstellar war, Star Force has to learn on the fly how to fight as the underdog against a technologically superior alien enemy that they know absolutely nothing about. Star Force: Counterstrike SF18 Choosing to widen the battlefield on Corneria to two fronts, Star Force sends three Clans to the planet's polar region to stake out a foothold on the snowy plains where their mechs can be brought into play